Aid stations are stocked with water, GU, GU2O, cookies, candy, boiled potatoes, chips, peanut butter and jelly sandwiches, pretzels, salt, bananas and melons. There will be soup in the evening at Tennessee Valley. Runners must carry a water bottle/hydration pack during the race.
| Aid Station | Distance (total) | Distance (to next aid station) |
| Bunker Road (water only) | 6.2 | 5.7 |
| Tennessee Valley | 11.9 | 4.1 |
| Muir Beach | 16.0 | 5.7 |
| Pan Toll | 21.7 | 6.7 |
| Bolinas Ridge | 28.4 | 7.2 |
| Randall Trail (turnaround) | 35.6 | 7.2 |
| Bolinas Ridge | 42.8 | 6.7 |
| Pan Toll | 49.5 | 5.2 |
| Highway 1 crossing | 54.7 | 3.7 |
| Tennessee Valley | 58.4 | 3.8 |
| Rodeo Lagoon | 62.4 | Finish |
